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Addgene inc aav serotype 8 hsyn flex hm3dq mcherry
Aav Serotype 8 Hsyn Flex Hm3dq Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 593 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav+serotype+8+hsyn+flex+hm3dq+mcherry/pAAV-hSyn-DIO-mCherry+(Plasmid+%2350459)/bio_rxiv__64898__2026__02__26__708360-100-17-7
Average 96 stars, based on 593 article reviews

aav serotype 8 hsyn flex hm3dq mcherry - by Bioz Stars, 2026-10

96/100 stars

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Bioprocessing:

Article Title: Artificially induced torpor during pregnancy impairs fetal growth in mice
Article Snippet: .. We obtained the following AAV vectors from Addgene (titer is shown as genome particles [gp] per mL): AAV serotype 8 hSyn-FLEx-hM3Dq-mCherry (#44361, 2.1 × 10 13 gp/mL), AAV serotype 8 hSyn-FLEx-mCherry (#50459, 1.7 × 10 13 gp/mL), AAV serotype 5 EF1a-FLEx-hChR2(H134R)-EYFP (#20298, 1.8 × 10 13 gp/mL), and AAV retrograde EF1a-DIO-FLPo (#87306, 2.3 × 10 13 gp/mL). .. AAV serotype 8 hSyn-fDIO-hM3Dq-mCherry (2.0 × 10 13 gp/mL) was purchased from the Canadian Neurophotonics Platform Viral Vector Core Facility.

Article Title: A neuronal population in the bed nucleus of the stria terminalis mediating pup-directed aggression in male mice
Article Snippet: .. We obtained the following AAV vectors from Addgene (titer is shown as genome particles [gp] per mL): AAV serotype 8 hSyn-FLEx-hM3Dq-mCherry (#44361, 3.2 × 10 13 gp/mL), AAV serotype 8 hSyn-FLEx-mCherry (#50459, 2.3 × 10 13 gp/mL), and AAV retrograde EF1a-FLPo (#55637, 2.2 × 10 13 gp/mL). .. AAV serotype 2 EF1a-FLEx-taCasp3-TEVp (3.4 × 10 12 gp/mL) and AAV serotype 5 Ef1a-fDIO-ChR2(H134R)-eYFP (1.0 × 10 13 gp/mL) were purchased from the University of North Carolina viral core and the Canadian Neurophotonics Platform viral vector core facility, respectively.

Article Title: Plasticity of Neural Connections Underlying Oxytocin-mediated Parental Behaviors of Male Mice
Article Snippet: .. We obtained the following AAV vectors from Addgene (titer is shown as genome particles [gp] per ml): AAV serotype 9 hSyn-Cre (#105555, 2.3 × 10 13 gp/ml), AAV serotype 8 hSyn-FLEx-hM3Dq-mCherry (#44361, 3.2 × 10 13 gp/ml), AAV serotype 8 hSyn-FLEx-mCherry (#50459, 2.3 × 10 13 gp/ml), and AAV serotype 5 EF1a-FLEx-hChR2(H134R)-EYFP (#20298, 7.7 × 10 12 gp/ml). ..

Image Search Results

(a) Schematic of the virus injection. AAV8-FLEx-hM3Dq-mCherry was injected into the bilateral PVH of AVP-Cre virgin male mice. (b) Schematic of the time line of the experiment. (c) Left, representative coronal section of the PVH. Magenta, hM3Dq-mCherry, blue, DAPI. Scale bar, 50 μm. Right, the number of hM3Dq+ neurons. n = 8 mice each. (d) Percentage of virgin males showing attack, ignore, or retrieve (*p < 0.05, two-tailed Fisher’s exact test). (e) The parental care duration of CNO-injected mice was longer than that of saline-injected males, but did not reach the level of statistical significance (p = 0.086, two-tailed Welch’s t -test). (f) Parental score (*p < 0.05, two-tailed Mann–Whitney U -test). (g) Cumulative probability of pup-directed aggression. The p-value is shown in the panel (Kolmogorov–Smirnov test). (h) Schematic of the virus injection. AAV5-FLEx-ChR2(H134R)-eYFP was injected into the bilateral PVH of AVP-Cre virgin male mice. An optical fiber was further inserted into the PVH. (i) Representative coronal brain section showing the location of the optical fiber and expression of ChR2-eYFP (green) in the PVH. Blue, DAPI. Scale bar, 50 μm. (j) Schematic of the time line of the experiment. Blue bar, blue LED stimulation pulsed at 10 Hz. (k) Percentage of virgin males showing attack, ignore, or retrieve. n = 5 each. (l) Parental care duration (*p < 0.05, one-way ANOVA with post hoc Tukey’s HSD). 30-min-illumination evoked a longer parental care duration, although statistical significance was only found in the Off condition. (m) Parental score. Error bars, SEM. See for more data.

Journal: bioRxiv

Article Title: Vasopressin-to-Oxytocin Receptor Crosstalk in the Preoptic Area Underlying Parental Behaviors in Male Mice

doi: 10.1101/2024.07.01.601605

Figure Lengend Snippet: (a) Schematic of the virus injection. AAV8-FLEx-hM3Dq-mCherry was injected into the bilateral PVH of AVP-Cre virgin male mice. (b) Schematic of the time line of the experiment. (c) Left, representative coronal section of the PVH. Magenta, hM3Dq-mCherry, blue, DAPI. Scale bar, 50 μm. Right, the number of hM3Dq+ neurons. n = 8 mice each. (d) Percentage of virgin males showing attack, ignore, or retrieve (*p < 0.05, two-tailed Fisher’s exact test). (e) The parental care duration of CNO-injected mice was longer than that of saline-injected males, but did not reach the level of statistical significance (p = 0.086, two-tailed Welch’s t -test). (f) Parental score (*p < 0.05, two-tailed Mann–Whitney U -test). (g) Cumulative probability of pup-directed aggression. The p-value is shown in the panel (Kolmogorov–Smirnov test). (h) Schematic of the virus injection. AAV5-FLEx-ChR2(H134R)-eYFP was injected into the bilateral PVH of AVP-Cre virgin male mice. An optical fiber was further inserted into the PVH. (i) Representative coronal brain section showing the location of the optical fiber and expression of ChR2-eYFP (green) in the PVH. Blue, DAPI. Scale bar, 50 μm. (j) Schematic of the time line of the experiment. Blue bar, blue LED stimulation pulsed at 10 Hz. (k) Percentage of virgin males showing attack, ignore, or retrieve. n = 5 each. (l) Parental care duration (*p < 0.05, one-way ANOVA with post hoc Tukey’s HSD). 30-min-illumination evoked a longer parental care duration, although statistical significance was only found in the Off condition. (m) Parental score. Error bars, SEM. See for more data.

Article Snippet: We obtained the following AAV vectors from Addgene (titer is shown as genome particles [gp] per ml): AAV serotype 9 hSyn-Cre (#105555, 2.3 × 10 13 gp/ml), AAV serotype 8 hSyn-FLEx-hM3Dq-mCherry (#44361, 3.2 × 10 13 gp/ml), AAV serotype 8 hSyn-FLEx-mCherry (#50459, 1.7 × 10 13 gp/ml), AAV serotype 5 Ef1a-FLEx-eYFP (#27056, 1.3 × 10 13 gp/ml), and AAV serotype 5 Ef1a-FLEx-hChR2(H134R)-eYFP (#20298, 2.1 × 10 13 gp/ml).

Techniques: Virus, Injection, Two Tailed Test, Saline, MANN-WHITNEY, Expressing

(a) Schematic of the virus injection. (b–d) Representative coronal sections of AVP-Cre virgin males expressing hM3Dq who interacted with isolated pups in a metal strainer (see Methods). Green represents c-fos mRNA. Magenta represents Galanin mRNA in the MPNm (b), Calcr mRNA in the MPNm (c), or Ucn3 mRNA in PeFA (d) in situ staining (*p < 0.05, **p < 0.01, two-tailed Welch’s t -test). n = 6 each. Blue, DAPI. Scale bar, 30 μm. (e) Schematic of the virus injection. AAV5-FLEx-eYFP was injected into the bilateral PVH. An optical fiber was further inserted into the PVH. The experimental procedures are the same as those in . (f) Percentage of virgin males showing attack, ignore, or retrieve. n = 5 mice each. (g) Parental care duration. (h) Parental score. (i) Schematic of the experiment. AAV-FLEx-hM3Dq-mCherry was injected into the bilateral PVH of OT-Cre mice. OTA, OTR antagonist, V1aA, V1aR antagonist. (j) The number of neurons expressing hM3Dq was not statistically different (p > 0.87, one-way ANOVA). Ip injection of saline, n = 6, 6, and 5 for saline, OTA, and V1aA, respectively. Ip injection of CNO, n = 7 mice each. (k) Percentage of animals showing attack, ignore, or retrieve. (l) Parental care duration (**p < 0.01, one-way ANOVA with post hoc Tukey’s HSD). (m) Parental score was not significantly different (saline, p > 0.46, CNO, p = 0.05, Kruskal-Wallis test). (n) Cumulative probability of pup-directed aggression (***p < 0.001, Kolmogorov–Smirnov test with Bonferroni correction). Error bars, SEM.

Journal: bioRxiv

Article Title: Vasopressin-to-Oxytocin Receptor Crosstalk in the Preoptic Area Underlying Parental Behaviors in Male Mice

doi: 10.1101/2024.07.01.601605

Figure Lengend Snippet: (a) Schematic of the virus injection. (b–d) Representative coronal sections of AVP-Cre virgin males expressing hM3Dq who interacted with isolated pups in a metal strainer (see Methods). Green represents c-fos mRNA. Magenta represents Galanin mRNA in the MPNm (b), Calcr mRNA in the MPNm (c), or Ucn3 mRNA in PeFA (d) in situ staining (*p < 0.05, **p < 0.01, two-tailed Welch’s t -test). n = 6 each. Blue, DAPI. Scale bar, 30 μm. (e) Schematic of the virus injection. AAV5-FLEx-eYFP was injected into the bilateral PVH. An optical fiber was further inserted into the PVH. The experimental procedures are the same as those in . (f) Percentage of virgin males showing attack, ignore, or retrieve. n = 5 mice each. (g) Parental care duration. (h) Parental score. (i) Schematic of the experiment. AAV-FLEx-hM3Dq-mCherry was injected into the bilateral PVH of OT-Cre mice. OTA, OTR antagonist, V1aA, V1aR antagonist. (j) The number of neurons expressing hM3Dq was not statistically different (p > 0.87, one-way ANOVA). Ip injection of saline, n = 6, 6, and 5 for saline, OTA, and V1aA, respectively. Ip injection of CNO, n = 7 mice each. (k) Percentage of animals showing attack, ignore, or retrieve. (l) Parental care duration (**p < 0.01, one-way ANOVA with post hoc Tukey’s HSD). (m) Parental score was not significantly different (saline, p > 0.46, CNO, p = 0.05, Kruskal-Wallis test). (n) Cumulative probability of pup-directed aggression (***p < 0.001, Kolmogorov–Smirnov test with Bonferroni correction). Error bars, SEM.

Techniques: Virus, Injection, Expressing, Isolation, In Situ, Staining, Two Tailed Test, Saline

(a) Schematic of the experiment. AAV-FLEx-hM3Dq-mCherry was injected into the bilateral PVH of AVP-Cre virgin male mice. OTR antagonist (OTA) or V1aR antagonist (V1aA) was injected into the ventricle through the cannula. (b) Representative coronal section showing the position of the cannula. Blue, DAPI. Scale bar, 500 μm. (c) Schematic of the time line of the experiment. Icv injection of OTA or V1aA was performed under isoflurane anesthesia. Note that the icv injection took approximately 3 min (Methods). (d) The number of neurons expressing hM3Dq was not statistically different (p > 0.46, one-way ANOVA). n = 5 each for ip injection of saline, n = 7 each for ip injection of CNO. (e) Percentage of animals showing attack, ignore, or retrieve. (f) Parental care duration (**p < 0.01, one-way ANOVA with post hoc Tukey’s HSD). (g) Parental score was not significantly different (saline, p > 0.79, CNO, p > 0.06, Kruskal-Wallis test). (h) Cumulative probability of pup-directed aggression (***p < 0.001, Kolmogorov–Smirnov test with Bonferroni correction). Error bars, SEM. See and for more data.

Journal: bioRxiv

Article Title: Vasopressin-to-Oxytocin Receptor Crosstalk in the Preoptic Area Underlying Parental Behaviors in Male Mice

doi: 10.1101/2024.07.01.601605

Figure Lengend Snippet: (a) Schematic of the experiment. AAV-FLEx-hM3Dq-mCherry was injected into the bilateral PVH of AVP-Cre virgin male mice. OTR antagonist (OTA) or V1aR antagonist (V1aA) was injected into the ventricle through the cannula. (b) Representative coronal section showing the position of the cannula. Blue, DAPI. Scale bar, 500 μm. (c) Schematic of the time line of the experiment. Icv injection of OTA or V1aA was performed under isoflurane anesthesia. Note that the icv injection took approximately 3 min (Methods). (d) The number of neurons expressing hM3Dq was not statistically different (p > 0.46, one-way ANOVA). n = 5 each for ip injection of saline, n = 7 each for ip injection of CNO. (e) Percentage of animals showing attack, ignore, or retrieve. (f) Parental care duration (**p < 0.01, one-way ANOVA with post hoc Tukey’s HSD). (g) Parental score was not significantly different (saline, p > 0.79, CNO, p > 0.06, Kruskal-Wallis test). (h) Cumulative probability of pup-directed aggression (***p < 0.001, Kolmogorov–Smirnov test with Bonferroni correction). Error bars, SEM. See and for more data.

Techniques: Injection, Expressing, Saline

(a) Schematic of the virus injection. AVP neurons express hM3Dq-mCherry while OT neurons express GCaMP driven by OT promotor (OTp) . (b) Schematic of the time line of the experiment. (c) Representative coronal brain section showing the location of the optical fiber and expression of GCaMP (green) and hM3Dq-mCherry (magenta) in the PVH. Blue, DAPI. Scale bar, 50 μm. (d) Sample recording from OT neurons in response to a tail pinch (arrow). (e) Sample recording from OT neurons with ip injection of saline or CNO. (f) The normalized area under the curve was not affected by CNO application (n = 4). (g) Schematic of the experiment. Saline containing AVP or saline alone was injected into the ventricle of wild-type ( OT +/+ ) or OT KO ( OT −/− ) virgin male mice. (h) Schematic of the time line of the experiment. (i) Percentage of animals showing attack, ignore, or retrieve (n = 5 and 6 for saline and AVP in OT +/+ , respectively; n = 6 each in OT −/− ). (j) Parental care duration. (k) Parental score (*p < 0.05, two-tailed Mann–Whitney U -test). (l) Cumulative probability of pup-directed aggression. The p-value is shown in the panel (Kolmogorov–Smirnov test). Error bars, SEM.

Journal: bioRxiv

Article Title: Vasopressin-to-Oxytocin Receptor Crosstalk in the Preoptic Area Underlying Parental Behaviors in Male Mice

doi: 10.1101/2024.07.01.601605

Figure Lengend Snippet: (a) Schematic of the virus injection. AVP neurons express hM3Dq-mCherry while OT neurons express GCaMP driven by OT promotor (OTp) . (b) Schematic of the time line of the experiment. (c) Representative coronal brain section showing the location of the optical fiber and expression of GCaMP (green) and hM3Dq-mCherry (magenta) in the PVH. Blue, DAPI. Scale bar, 50 μm. (d) Sample recording from OT neurons in response to a tail pinch (arrow). (e) Sample recording from OT neurons with ip injection of saline or CNO. (f) The normalized area under the curve was not affected by CNO application (n = 4). (g) Schematic of the experiment. Saline containing AVP or saline alone was injected into the ventricle of wild-type ( OT +/+ ) or OT KO ( OT −/− ) virgin male mice. (h) Schematic of the time line of the experiment. (i) Percentage of animals showing attack, ignore, or retrieve (n = 5 and 6 for saline and AVP in OT +/+ , respectively; n = 6 each in OT −/− ). (j) Parental care duration. (k) Parental score (*p < 0.05, two-tailed Mann–Whitney U -test). (l) Cumulative probability of pup-directed aggression. The p-value is shown in the panel (Kolmogorov–Smirnov test). Error bars, SEM.

Techniques: Virus, Injection, Expressing, Saline, Two Tailed Test, MANN-WHITNEY

(a) Schematic of the experiment. AVP-Cre/+; OTR flox/flox mice were used for the experiment. AAV-Cre was injected into the POA or “posterior” hypothalamus while AAV-FLEx-hM3Dq-mCherry was injected into the bilateral PVH. (b) Representative coronal sections. Cre in situ staining is shown in green. Blue, DAPI. Scale bar, 50 μm. (c) Number of hM3Dq+ neurons in the PVH (n = 6 each). Mice that had 100 or more hM3Dq+ neurons were used. (d) Percentage of animals showing attack, ignore, or retrieve. (e) Parental care duration. (f) Parental score (*p < 0.05, two-tailed Mann–Whitney U -test). (g) Cumulative probability of pup-directed aggression. The p-value is shown in the panel (Kolmogorov–Smirnov test). Error bars, SEM.

Journal: bioRxiv

Article Title: Vasopressin-to-Oxytocin Receptor Crosstalk in the Preoptic Area Underlying Parental Behaviors in Male Mice

doi: 10.1101/2024.07.01.601605

Figure Lengend Snippet: (a) Schematic of the experiment. AVP-Cre/+; OTR flox/flox mice were used for the experiment. AAV-Cre was injected into the POA or “posterior” hypothalamus while AAV-FLEx-hM3Dq-mCherry was injected into the bilateral PVH. (b) Representative coronal sections. Cre in situ staining is shown in green. Blue, DAPI. Scale bar, 50 μm. (c) Number of hM3Dq+ neurons in the PVH (n = 6 each). Mice that had 100 or more hM3Dq+ neurons were used. (d) Percentage of animals showing attack, ignore, or retrieve. (e) Parental care duration. (f) Parental score (*p < 0.05, two-tailed Mann–Whitney U -test). (g) Cumulative probability of pup-directed aggression. The p-value is shown in the panel (Kolmogorov–Smirnov test). Error bars, SEM.

Techniques: Injection, In Situ, Staining, Two Tailed Test, MANN-WHITNEY

(a) Schematic of the virus injection. AVP-Cre/+; OTR flox/flox virgin males were used for the experiment. AAV8-FLEx-hM3Dq-mCherry was injected into the bilateral PVH to activate AVP neurons chemogenetically. AAV2-Cre was further injected into the bilateral MPNm or ACN to perform cKO of OTR . The AAVs were injected as depicted on the time line. (b) Representative coronal sections of the MPNm (top) or ACN (bottom). Cre in situ staining is shown in green. Blue, DAPI. MPNl, lateral part of the medial preoptic nucleus. Scale bar, 50 μm. (c) Representative coronal sections showing the MPNm and ACN from a saline-injected mouse. OTR mRNA was visualized by RNAscope (magenta). Blue, DAPI. Scale bar, 30 μm. (d) Number of neurons expressing hM3Dq in the PVH. Mice that had 100 or more hM3Dq+ neurons were used for this experiment as they show a higher ratio of retrieval. Top, those mice received AAV-Cre injection into the MPNm; bottom, into the ACN. MPNm, n = 7 mice each; ACN, n = 6 and 7 mice for saline and +Cre, respectively. (e) Fraction of DAPI+ cells expressing OTR (***p < 0.001, two-tailed Welch’s t -test). (f) Percentage of virgin males showing attack, ignore, or retrieve. Of note, although OTR in AVP neurons might be deleted in this experimental condition, CNO injection consistently facilitated paternal behaviors in mice that received saline injection into the MPNm or ACN. (g) Parental care duration (*p < 0.05, two-tailed Welch’s t -test). (h) Parental score. (i) Cumulative probability of pup-directed aggression and pup retrieval. The p-value is shown in the panel (Kolmogorov–Smirnov test). Error bars, SEM. See and for more data.

Journal: bioRxiv

Article Title: Vasopressin-to-Oxytocin Receptor Crosstalk in the Preoptic Area Underlying Parental Behaviors in Male Mice

doi: 10.1101/2024.07.01.601605

Figure Lengend Snippet: (a) Schematic of the virus injection. AVP-Cre/+; OTR flox/flox virgin males were used for the experiment. AAV8-FLEx-hM3Dq-mCherry was injected into the bilateral PVH to activate AVP neurons chemogenetically. AAV2-Cre was further injected into the bilateral MPNm or ACN to perform cKO of OTR . The AAVs were injected as depicted on the time line. (b) Representative coronal sections of the MPNm (top) or ACN (bottom). Cre in situ staining is shown in green. Blue, DAPI. MPNl, lateral part of the medial preoptic nucleus. Scale bar, 50 μm. (c) Representative coronal sections showing the MPNm and ACN from a saline-injected mouse. OTR mRNA was visualized by RNAscope (magenta). Blue, DAPI. Scale bar, 30 μm. (d) Number of neurons expressing hM3Dq in the PVH. Mice that had 100 or more hM3Dq+ neurons were used for this experiment as they show a higher ratio of retrieval. Top, those mice received AAV-Cre injection into the MPNm; bottom, into the ACN. MPNm, n = 7 mice each; ACN, n = 6 and 7 mice for saline and +Cre, respectively. (e) Fraction of DAPI+ cells expressing OTR (***p < 0.001, two-tailed Welch’s t -test). (f) Percentage of virgin males showing attack, ignore, or retrieve. Of note, although OTR in AVP neurons might be deleted in this experimental condition, CNO injection consistently facilitated paternal behaviors in mice that received saline injection into the MPNm or ACN. (g) Parental care duration (*p < 0.05, two-tailed Welch’s t -test). (h) Parental score. (i) Cumulative probability of pup-directed aggression and pup retrieval. The p-value is shown in the panel (Kolmogorov–Smirnov test). Error bars, SEM. See and for more data.

Techniques: Virus, Injection, In Situ, Staining, Saline, RNAscope, Expressing, Two Tailed Test

(a) Schematic of the virus injection and experimental time line. AAV8-FLEx-hM3Dq-mCherry was injected into the bilateral PVH, while AAV2-Cre was injected into the bilateral MPNm or ACN of OT-Cre/+; OTR flox/flox virgin males. (b) Number of neurons expressing hM3Dq in the PVH. Top, data from the mice who received AAV-Cre injection into the MPNm; bottom, into the ACN. MPNm, n = 7 and 6 for saline and +Cre, respectively; ACN, n = 7 and 6 for saline and +Cre, respectively. (c) Fraction of DAPI+ cells expressing OTR (***p < 0.001, two-tailed Welch’s t -test). (d) Percentage of virgin males showing attack, ignore, or retrieve. Of note, OTR may be deleted in OT neurons in this experimental condition, which did not affect the induction of pup retrieval upon CNO injection in mice that received saline injection into the MPNm or ACN. (e) Parental care duration. (f) Parental score. (g) Cumulative probability of pup-directed aggression and pup retrieval. The p-value is shown in the panel (Kolmogorov–Smirnov test). Error bars, SEM.

Journal: bioRxiv

Article Title: Vasopressin-to-Oxytocin Receptor Crosstalk in the Preoptic Area Underlying Parental Behaviors in Male Mice

doi: 10.1101/2024.07.01.601605

Figure Lengend Snippet: (a) Schematic of the virus injection and experimental time line. AAV8-FLEx-hM3Dq-mCherry was injected into the bilateral PVH, while AAV2-Cre was injected into the bilateral MPNm or ACN of OT-Cre/+; OTR flox/flox virgin males. (b) Number of neurons expressing hM3Dq in the PVH. Top, data from the mice who received AAV-Cre injection into the MPNm; bottom, into the ACN. MPNm, n = 7 and 6 for saline and +Cre, respectively; ACN, n = 7 and 6 for saline and +Cre, respectively. (c) Fraction of DAPI+ cells expressing OTR (***p < 0.001, two-tailed Welch’s t -test). (d) Percentage of virgin males showing attack, ignore, or retrieve. Of note, OTR may be deleted in OT neurons in this experimental condition, which did not affect the induction of pup retrieval upon CNO injection in mice that received saline injection into the MPNm or ACN. (e) Parental care duration. (f) Parental score. (g) Cumulative probability of pup-directed aggression and pup retrieval. The p-value is shown in the panel (Kolmogorov–Smirnov test). Error bars, SEM.

Techniques: Virus, Injection, Expressing, Saline, Two Tailed Test

(a) Schematic of the virus injection. Presynaptic neurons of OTR+ neurons in the MPNm (top) or ACN (bottom) are labeled by GFP expression. (b, c) Representative coronal sections of the PVH with transsynaptic tracing from the MPNm (top) or ACN (bottom). Green represents in situ staining of GFP . Magenta represents in situ staining of OT (b) or AVP (c). Blue, DAPI. Scale bar, 30 μm. (d) Percentage of dual-positive neurons among GFP+ neurons in the PVH. In presynaptic neurons among OTR+ neurons in the MPNm (top) and OTR+ neurons in the ACN (bottom), no significant difference was found between OT+ and AVP+ (p > 0.93 and p > 0.56 for MPNm and ACN, respectively, Two-tailed Mann–Whitney U -test. MPNm, n = 6 each; ACN, n = 7 and 6 for OT+ and AVP+ , respectively). (e) Schematic of the virus injection. AAV8-FLEx-mCherry was injected into the bilateral ACN of OTR-iCre virgin males. (f) Representative coronal sections of the ACN. Green shows vGluT2 (left) or vGAT (right) in situ staining. Magenta, mCherry. Blue, DAPI. Scale bar, 50 μm. (g) Fraction of mCherry+ neurons co-expressing vGluT2 or vGAT (n = 5 mice; ***p < 0.001, paired t -test). (h) Representative coronal sections of the ACN showing SST (left), Etv1 (middle), or Tac2 (right) mRNA (green). Magenta, mCherry. Blue, DAPI. Scale bar, 50 μm. (i) Fraction of mCherry+ neurons co-expressing SST , Etv1 , or Tac2 (n = 9 mice; **p < 0.01, one-way ANOVA with a post hoc paired t -test with Bonferroni correction). (j) Summary of behavioral phenotypes. (k) Graphical abstract.

Journal: bioRxiv

Article Title: Vasopressin-to-Oxytocin Receptor Crosstalk in the Preoptic Area Underlying Parental Behaviors in Male Mice

doi: 10.1101/2024.07.01.601605

Figure Lengend Snippet: (a) Schematic of the virus injection. Presynaptic neurons of OTR+ neurons in the MPNm (top) or ACN (bottom) are labeled by GFP expression. (b, c) Representative coronal sections of the PVH with transsynaptic tracing from the MPNm (top) or ACN (bottom). Green represents in situ staining of GFP . Magenta represents in situ staining of OT (b) or AVP (c). Blue, DAPI. Scale bar, 30 μm. (d) Percentage of dual-positive neurons among GFP+ neurons in the PVH. In presynaptic neurons among OTR+ neurons in the MPNm (top) and OTR+ neurons in the ACN (bottom), no significant difference was found between OT+ and AVP+ (p > 0.93 and p > 0.56 for MPNm and ACN, respectively, Two-tailed Mann–Whitney U -test. MPNm, n = 6 each; ACN, n = 7 and 6 for OT+ and AVP+ , respectively). (e) Schematic of the virus injection. AAV8-FLEx-mCherry was injected into the bilateral ACN of OTR-iCre virgin males. (f) Representative coronal sections of the ACN. Green shows vGluT2 (left) or vGAT (right) in situ staining. Magenta, mCherry. Blue, DAPI. Scale bar, 50 μm. (g) Fraction of mCherry+ neurons co-expressing vGluT2 or vGAT (n = 5 mice; ***p < 0.001, paired t -test). (h) Representative coronal sections of the ACN showing SST (left), Etv1 (middle), or Tac2 (right) mRNA (green). Magenta, mCherry. Blue, DAPI. Scale bar, 50 μm. (i) Fraction of mCherry+ neurons co-expressing SST , Etv1 , or Tac2 (n = 9 mice; **p < 0.01, one-way ANOVA with a post hoc paired t -test with Bonferroni correction). (j) Summary of behavioral phenotypes. (k) Graphical abstract.

Techniques: Virus, Injection, Labeling, Expressing, In Situ, Staining, Two Tailed Test, MANN-WHITNEY

(a) Schematic of the virus injection. AAV8-FLEx-hM3Dq-mCherry was injected into the bilateral MPNm or ACN of OTR-iCre virgin males. (b) Schematic of the time line of the experiment. (c) Representative coronal sections of the MPNm (left) or ACN (right). Magenta, hM3Dq-mCherry, Blue, DAPI. MPNl, lateral part of the medial preoptic nucleus. Scale bar, 50 μm. (d) Number of hM3Dq+ neurons. MPNm, n = 7 and 6 for saline and CNO, respectively; ACN, n = 6 each. (e) Percentage of males showing attack, ignore, or retrieve (*p < 0.05, **p < 0.01, two-tailed Fisher’s exact test). (f) Parental care duration (*p < 0.05, two-tailed Welch’s t -test). (g) Parental score (*p < 0.05, **p < 0.01, two-tailed Mann–Whitney U -test). (h) Cumulative probability of pup-directed aggression and pup retrieval. The p-value is shown in the panel (Kolmogorov–Smirnov test). Error bars, SEM. See for more data.

Journal: bioRxiv

Article Title: Vasopressin-to-Oxytocin Receptor Crosstalk in the Preoptic Area Underlying Parental Behaviors in Male Mice

doi: 10.1101/2024.07.01.601605

Figure Lengend Snippet: (a) Schematic of the virus injection. AAV8-FLEx-hM3Dq-mCherry was injected into the bilateral MPNm or ACN of OTR-iCre virgin males. (b) Schematic of the time line of the experiment. (c) Representative coronal sections of the MPNm (left) or ACN (right). Magenta, hM3Dq-mCherry, Blue, DAPI. MPNl, lateral part of the medial preoptic nucleus. Scale bar, 50 μm. (d) Number of hM3Dq+ neurons. MPNm, n = 7 and 6 for saline and CNO, respectively; ACN, n = 6 each. (e) Percentage of males showing attack, ignore, or retrieve (*p < 0.05, **p < 0.01, two-tailed Fisher’s exact test). (f) Parental care duration (*p < 0.05, two-tailed Welch’s t -test). (g) Parental score (*p < 0.05, **p < 0.01, two-tailed Mann–Whitney U -test). (h) Cumulative probability of pup-directed aggression and pup retrieval. The p-value is shown in the panel (Kolmogorov–Smirnov test). Error bars, SEM. See for more data.

Techniques: Virus, Injection, Saline, Two Tailed Test, MANN-WHITNEY

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